A rapid and cost-effective method for sequencing pooled cDNA clones by using a combination of transposon insertion and Gateway technology

Takeya Morozumi; Daisuke Toki; Tomoko Eguchi-Ogawa; Hirohide Uenishi

doi:10.2144/000113737

A rapid and cost-effective method for sequencing pooled cDNA clones by using a combination of transposon insertion and Gateway technology

Biotechniques. 2011 Sep;51(3):195-7. doi: 10.2144/000113737.

Authors

Takeya Morozumi¹, Daisuke Toki, Tomoko Eguchi-Ogawa, Hirohide Uenishi

Affiliation

¹ Animal Genome Research Program, Animal Research Division, Institute of Society for Techno-innovation of Agriculture, Forestry and Fisheries, Tsukuba, Japan.

PMID: 21906043
DOI: 10.2144/000113737

Abstract

Large-scale cDNA-sequencing projects require an efficient strategy for mass sequencing. Here we describe a method for sequencing pooled cDNA clones using a combination of transposon insertion and Gateway technology. Our method reduces the number of shotgun clones that are unsuitable for reconstruction of cDNA sequences, and has the advantage of reducing the total costs of the sequencing project.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cost-Benefit Analysis
DNA Transposable Elements / genetics
DNA, Complementary / chemistry*
DNA, Complementary / genetics*
Gene Library
High-Throughput Nucleotide Sequencing / economics
High-Throughput Nucleotide Sequencing / methods*
Mutagenesis, Insertional / methods*
Swine / genetics

Substances

DNA Transposable Elements
DNA, Complementary