Cell labeling approaches for fluorescence-based in vivo flow cytometry

Cytometry A. 2011 Oct;79(10):758-65. doi: 10.1002/cyto.a.21125. Epub 2011 Sep 8.

Abstract

We provide an overview of the methods used to label circulating cells for fluorescence detection by in vivo flow cytometry. These methods are useful for cell tracking in small animals without the need to draw blood samples and are particularly useful for the detection of circulating cancer cells and quantification of circulating immune cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Adoptive Transfer
  • Animals
  • Antibodies / analysis*
  • Antibodies / metabolism
  • Cell Line, Tumor
  • Flow Cytometry* / methods
  • Fluorescence
  • Fluorescent Dyes / analysis*
  • Fluorescent Dyes / chemistry
  • Green Fluorescent Proteins / analysis*
  • Green Fluorescent Proteins / chemistry
  • Humans
  • Mice
  • Molecular Imaging / methods
  • Neoplasms / diagnosis*
  • Neoplasms / pathology
  • Neoplastic Cells, Circulating / pathology*
  • Photoacoustic Techniques / methods
  • Staining and Labeling* / methods

Substances

  • Antibodies
  • Fluorescent Dyes
  • Green Fluorescent Proteins