Simultaneous "one pot" expressed protein ligation and CuI-catalyzed azide/alkyne cycloaddition for protein immobilization

Chembiochem. 2011 Nov 4;12(16):2426-30. doi: 10.1002/cbic.201100434. Epub 2011 Sep 8.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkynes / chemistry*
  • Azides / chemistry*
  • Catalysis
  • Click Chemistry
  • Copper / chemistry*
  • Green Fluorescent Proteins / chemistry
  • Green Fluorescent Proteins / metabolism
  • Immobilized Proteins / chemistry*
  • Immobilized Proteins / metabolism
  • Microscopy, Fluorescence

Substances

  • Alkynes
  • Azides
  • Immobilized Proteins
  • enhanced green fluorescent protein
  • Green Fluorescent Proteins
  • Copper