Abstract
An aqueous enzymatic process assisted by ultrasound extraction (AEP-UE) was applied to the extraction of oil from flaxseed (Linum usitatissimum L.). The highest oil recovery of 68.1% was obtained when ground flaxseed was incubated with 130 U/g of cellulase, pectinase, and hemicellulase for 12h, at 45°C and pH 5.0. The IC(50) values of oil obtained by AEP-UE and organic solvent extraction (OSE), as measured by DPPH scavenging activity essay, were 2.27 mg/mL and 3.31 mg/mL. The AEP-UE-derived oil had a 1.5% higher content of unsaturated fatty acids than the OSE-derived oil. AEP-UE is therefore a promising environmentally friendly method for large-scale preparation of flaxseed oil.
Copyright © 2011 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biphenyl Compounds / metabolism
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Cellulase / metabolism
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Enzyme Stability / drug effects
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Enzymes, Immobilized / metabolism*
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Fatty Acids / analysis
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Free Radical Scavengers / pharmacology
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Gas Chromatography-Mass Spectrometry
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Glycoside Hydrolases / metabolism
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Hydrogen-Ion Concentration / drug effects
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Kinetics
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Linseed Oil / analysis
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Linseed Oil / chemistry
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Linseed Oil / isolation & purification*
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Linseed Oil / pharmacology
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Picrates / metabolism
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Polygalacturonase / metabolism
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Principal Component Analysis
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Temperature
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Time Factors
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Ultrasonics / methods*
Substances
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Biphenyl Compounds
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Enzymes, Immobilized
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Fatty Acids
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Free Radical Scavengers
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Picrates
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Linseed Oil
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1,1-diphenyl-2-picrylhydrazyl
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Glycoside Hydrolases
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hemicellulase
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Polygalacturonase
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Cellulase