Abstract
In the present study, we investigated the effect of the Kampo medicine Yokukansan (YKS) on pentobarbital-induced sleep in group-housed and socially isolated mice. Socially isolated mice showed shorter sleeping time than the group-housed mice. YKS (300 mg/kg, p.o.) prolonged the pentobarbital-induced sleeping time in socially isolated mice without affecting pentobarbital sleep in group-housed mice. The prolongation of sleeping time by YKS was reversed by bicuculline (3 mg/kg, i.p.) and flumazenil (3 mg/kg, i.p.), but not WAY100635. These findings suggest that the GABA(A)-benzodiazepine receptor complex, but not 5-HT(1A) receptors, is involved in the reversal effect of YKS on the decrease of pentobarbital sleep by social isolation.
MeSH terms
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Animals
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Bicuculline / pharmacology
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Dose-Response Relationship, Drug
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Drug Synergism
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Drugs, Chinese Herbal / administration & dosage
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Drugs, Chinese Herbal / therapeutic use*
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Flumazenil / pharmacology
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GABA-A Receptor Agonists / therapeutic use
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GABA-A Receptor Antagonists / pharmacology
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Hypnotics and Sedatives / therapeutic use*
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Male
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Medicine, Kampo
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Mice
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Mice, Inbred Strains
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Pentobarbital / therapeutic use*
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Receptors, GABA-A / chemistry
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Receptors, GABA-A / metabolism*
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Sleep / drug effects*
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Sleep Wake Disorders / drug therapy*
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Sleep Wake Disorders / etiology
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Sleep Wake Disorders / metabolism
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Social Isolation*
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Stress, Psychological / physiopathology
Substances
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Drugs, Chinese Herbal
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GABA-A Receptor Agonists
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GABA-A Receptor Antagonists
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Hypnotics and Sedatives
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Receptors, GABA-A
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Yi-Gan San
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Flumazenil
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Pentobarbital
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Bicuculline