Abstract
Cellular redox conditions affect Gsp amidase activity in Escherichia coli. Guided by the structure and catalytic mechanism of the amidase, we designed and synthesized an acyloxymethyl ketone-based activity probe containing a biotin handle. This probe was used to monitor Gsp amidase activity in E. coli lysates that had been subjected to oxidative or methylglyoxal-induced stress.
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amidohydrolases / metabolism*
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Binding Sites
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Enzyme Assays / methods*
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Escherichia coli / enzymology*
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Glutathione / analogs & derivatives*
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Glutathione / metabolism
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Ketones / chemistry
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Ketones / metabolism*
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Models, Molecular
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Oxidation-Reduction
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Spermidine / analogs & derivatives*
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Spermidine / metabolism
Substances
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Ketones
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glutathionylspermidine
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Amidohydrolases
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amidase
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Glutathione
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Spermidine