An acyloxymethyl ketone-based probe to monitor the activity of glutathionylspermidine amidase in Escherichia coli

Chembiochem. 2011 Oct 17;12(15):2306-9. doi: 10.1002/cbic.201100417. Epub 2011 Sep 2.

Abstract

Cellular redox conditions affect Gsp amidase activity in Escherichia coli. Guided by the structure and catalytic mechanism of the amidase, we designed and synthesized an acyloxymethyl ketone-based activity probe containing a biotin handle. This probe was used to monitor Gsp amidase activity in E. coli lysates that had been subjected to oxidative or methylglyoxal-induced stress.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amidohydrolases / metabolism*
  • Binding Sites
  • Enzyme Assays / methods*
  • Escherichia coli / enzymology*
  • Glutathione / analogs & derivatives*
  • Glutathione / metabolism
  • Ketones / chemistry
  • Ketones / metabolism*
  • Models, Molecular
  • Oxidation-Reduction
  • Spermidine / analogs & derivatives*
  • Spermidine / metabolism

Substances

  • Ketones
  • glutathionylspermidine
  • Amidohydrolases
  • amidase
  • Glutathione
  • Spermidine