[Effect of ciglitazone on THP-1 macrophage CD36 expression and cholesterol influx]

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2011 Jun;27(6):685-7.
[Article in Chinese]

Abstract

Aim: To investigate the effect of ciglitazone on CD36 expression and cholesterol influx in THP-1 macrophage.

Methods: After exposure of the cultured THP-1 macrophage to ciglitazone for 24 h, [(3)H] labeled Cholesterol influx was determined by FJ-2107P typed liquid scintillator. CD36 mRNA and protein level were determined by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting respectively.

Results: PPARĪ³ agonist, ciglitazone, elevated CD36 in both protein and mRNA levels, and increased cholesterol influx in THP-1 macrophage. The levels of cholesterol influx were 20. 3%, 28. 6%, 37. 2%, 44. 3%, 48. 7% respectively.

Conclusion: Our results indicated that ciglitazone may play an important role in cholesterol influx and modulating CD36 expression in THP-1 macrophage.

MeSH terms

  • CD36 Antigens / drug effects
  • CD36 Antigens / metabolism*
  • Cell Line
  • Cholesterol / metabolism*
  • Foam Cells / drug effects
  • Foam Cells / metabolism
  • Humans
  • Macrophages / drug effects*
  • Macrophages / metabolism*
  • Male
  • PPAR gamma / agonists
  • RNA, Messenger / drug effects
  • RNA, Messenger / metabolism
  • Thiazolidinediones / pharmacology*

Substances

  • CD36 Antigens
  • PPAR gamma
  • RNA, Messenger
  • Thiazolidinediones
  • Cholesterol
  • ciglitazone