Effects on transcription of mutations in ygjD, yeaZ, and yjeE genes, which are involved in a universal tRNA modification in Escherichia coli

Chika Hashimoto; Kohei Sakaguchi; Yuko Taniguchi; Hirofumi Honda; Taku Oshima; Naotake Ogasawara; Jun-Ichi Kato

doi:10.1128/JB.05733-11

Effects on transcription of mutations in ygjD, yeaZ, and yjeE genes, which are involved in a universal tRNA modification in Escherichia coli

J Bacteriol. 2011 Nov;193(21):6075-9. doi: 10.1128/JB.05733-11. Epub 2011 Aug 26.

Authors

Chika Hashimoto¹, Kohei Sakaguchi, Yuko Taniguchi, Hirofumi Honda, Taku Oshima, Naotake Ogasawara, Jun-Ichi Kato

Affiliation

¹ Department of Biology, Graduate School of Science, Tokyo Metropolitan University, 1-1 Minamiohsawa, Hachioji, Tokyo 192-0397, Japan.

Abstract

The Escherichia coli ygjD gene is critical for the universal tRNA modification N(6)-threonylcarbamoyladenosine, together with two other essential genes, yeaZ and yjeE. This study showed that the transcription of the thr and ilv operons in ygjD mutants was increased through the inhibition of transcription attenuation and that dnaG transcription was reduced.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Escherichia coli / genetics*
Escherichia coli / metabolism
Escherichia coli Proteins / genetics*
Mutation*
RNA, Transfer / metabolism*
Transcription, Genetic*

Substances

Escherichia coli Proteins
YeaZ protein, E coli
YgjD protein, E coli
YjeE protein, E coli
RNA, Transfer