Production of a novel biomacromolecule for nanodevices from glycerol as carbon source in different conditions

Int J Biol Macromol. 2011 Dec 1;49(5):955-7. doi: 10.1016/j.ijbiomac.2011.08.014. Epub 2011 Aug 19.

Abstract

The aim of this study was the investigation of producing cruxrhodopsin as a biomacromolecule with nanofunction from glycerol as carbon source using several process parameters. The optimum medium composition for cruxrhodopsin production was found to contain glycerol 1%, yeast extract 0.05% and K(2)HPO(4) 0.001%. The production of cruxrhodopsin in optimal conditions was 139.86 mg/l. In conclusion, halophilic microorganism Haloarcula sp. IRU1 could be a potential microorganism for production of cruxrhodopsin from glycerol in different conditions.

MeSH terms

  • Bacteriorhodopsins / isolation & purification
  • Bacteriorhodopsins / metabolism*
  • Bioreactors
  • Biotechnology / methods*
  • Carbon / metabolism
  • Cell Extracts
  • Culture Media
  • Fermentation
  • Glycerol / metabolism*
  • Haloarcula / chemistry
  • Haloarcula / enzymology*
  • Haloarcula / isolation & purification
  • Hydrogen-Ion Concentration
  • Nanostructures / chemistry*
  • Phosphates / metabolism
  • Potassium Compounds / metabolism
  • Yeasts / metabolism

Substances

  • Cell Extracts
  • Culture Media
  • Phosphates
  • Potassium Compounds
  • Bacteriorhodopsins
  • Carbon
  • potassium phosphate
  • Glycerol