Sperm caspase-9 in oligoasthenoteratozoospermic men with and without varicocele

Abstract

Objective: To assess sperm caspase-9 activity in infertile oligoasthenoteratozoospermic (OAT) men with and without varicocele.

Design: Prospective.

Setting: Academic setting.

Patient(s): Eighty men: healthy fertile control subjects (n = 20), OAT (n = 25), and OAT associated with left-side varicocele (n = 35).

Intervention(s): History taking, clinical examination, semen analysis,assessment of seminal caspase-9.

Main outcome measure(s): Semen analysis, sperm caspase-9.

Result(s): Sperm caspase-9 was significantly increased in infertile OAT men associated with varicocele compared with OAT men without varicocele and healthy fertile control subjects. Sperm casapse-9 activity demonstrated significant negative correlation with sperm count, sperm motility, sperm velocity, sperm linear velocity, sperm linearity index, and sperm normal morphology.

Conclusion(s): Sperm caspase-9 is exaggerated in infertile OAT cases with varicocele compared with infertile OAT cases without varicocele or healthy fertile men. Sperm caspase-9 demonstrated significant negative correlation with semen variables.