The study was purposed to investigate the effects of C-MYC siRNA on the proliferation and apoptosis of acute lymphoblastic Jurkat cell line. siRNA targeting the site 1545-1565 of C-MYC mRNA was designed and chemically synthesized, then C-MYC siRNA was transfected into Jurkat cells by the transfer agent (HiPerFect Transfection Reagent), the morphological changes were observed under inverted microscope; the tetrazole compound (MTS) was applied to draw the cell growth curve; the cell colony test was used to detect the effect of C-MYC siRNA on the proliferation of Jurkat cells; the flow cytometry and TUNEL method were used to analyze the apoptosis of Jurkat cells. The results showed that after Jurkat cells were treated with different concentrations of C-MYC siRNA, the growth of Jurkat cells was inhibited to various degrees, inhibitory rate was enhanced as C-MYC siRNA concentration increased. C-MYC siRNA also could obviously inhibit the cell clony formation. The apoptosis of cells could be detected by flow cytometry and TUNEL method, the apoptosis rate of cells increased along with prolonging of treatment with C-MYC siRNA. It is concluded that the chemically synthesized C-MYC siRNA can inhibit significantly the proliferation and induce the apoptosis of Jurkat cells.