A novel magnetic mesoporous silica packed S-shaped microfluidic reactor for online proteolysis of low-MW proteome

Chem Commun (Camb). 2011 Oct 14;47(38):10725-7. doi: 10.1039/c1cc13969j. Epub 2011 Aug 24.

Abstract

Magnetic mesoporous silica with a magnetic cover and mesoporous core was synthesized, filled with trypsin and located in an S-shaped microfluidic reactor. High-molecular weight (MW) proteins were split to waste by fractionation, whilst low-MW proteins were retained on the chip to be digested.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ferrosoferric Oxide / chemistry
  • Humans
  • Magnetics*
  • Microfluidic Analytical Techniques
  • Molecular Weight
  • Polyethylenes / chemistry
  • Porosity
  • Proteins / metabolism
  • Proteolysis
  • Proteome / metabolism*
  • Quaternary Ammonium Compounds / chemistry
  • Silicon Dioxide / chemistry*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Trypsin / metabolism

Substances

  • Polyethylenes
  • Proteins
  • Proteome
  • Quaternary Ammonium Compounds
  • SBA-15
  • poly-N,N-dimethyl-N,N-diallylammonium chloride
  • Silicon Dioxide
  • Trypsin
  • Ferrosoferric Oxide