Abstract
Magnetic mesoporous silica with a magnetic cover and mesoporous core was synthesized, filled with trypsin and located in an S-shaped microfluidic reactor. High-molecular weight (MW) proteins were split to waste by fractionation, whilst low-MW proteins were retained on the chip to be digested.
This journal is © The Royal Society of Chemistry 2011
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Ferrosoferric Oxide / chemistry
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Humans
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Magnetics*
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Microfluidic Analytical Techniques
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Molecular Weight
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Polyethylenes / chemistry
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Porosity
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Proteins / metabolism
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Proteolysis
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Proteome / metabolism*
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Quaternary Ammonium Compounds / chemistry
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Silicon Dioxide / chemistry*
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Trypsin / metabolism
Substances
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Polyethylenes
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Proteins
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Proteome
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Quaternary Ammonium Compounds
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SBA-15
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poly-N,N-dimethyl-N,N-diallylammonium chloride
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Silicon Dioxide
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Trypsin
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Ferrosoferric Oxide