Rational design of a cyclin A fluorescent peptide sensor

Org Biomol Chem. 2011 Oct 26;9(22):7629-32. doi: 10.1039/c1ob06009k. Epub 2011 Aug 23.

Abstract

We report the design and development of a fluorescent sensor specifically designed to target cyclin A, a protein that plays a key role in the regulation of the cell cycle. Computational studies provide a molecular picture that explains the observed emission increase, suggesting that the 4-DMAP fluorophore in the peptide is protected from the bulk solvent when inserted into the hydrophobic binding groove of cyclin A.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Biological Assay
  • Biosensing Techniques / methods*
  • Cell Cycle / drug effects
  • Cell Proliferation / drug effects
  • Cyclin A / antagonists & inhibitors
  • Cyclin A / chemistry*
  • Cyclin A / metabolism
  • Cyclin-Dependent Kinases / antagonists & inhibitors
  • Cyclin-Dependent Kinases / metabolism
  • Fluorescent Dyes / chemistry
  • Fluorescent Dyes / metabolism
  • HeLa Cells
  • Humans
  • Models, Molecular
  • Molecular Imprinting / methods*
  • Neoplasms / drug therapy
  • Neoplasms / metabolism*
  • Peptide Library
  • Peptides / chemical synthesis
  • Peptides / metabolism*
  • Phthalimides / chemistry*
  • Phthalimides / metabolism
  • Protein Binding
  • Protein Kinase Inhibitors / metabolism
  • Protein Kinase Inhibitors / pharmacology
  • Spectrometry, Fluorescence
  • Staining and Labeling / methods*

Substances

  • Cyclin A
  • Fluorescent Dyes
  • Peptide Library
  • Peptides
  • Phthalimides
  • Protein Kinase Inhibitors
  • Cyclin-Dependent Kinases