Abstract
We report the design and development of a fluorescent sensor specifically designed to target cyclin A, a protein that plays a key role in the regulation of the cell cycle. Computational studies provide a molecular picture that explains the observed emission increase, suggesting that the 4-DMAP fluorophore in the peptide is protected from the bulk solvent when inserted into the hydrophobic binding groove of cyclin A.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Binding Sites
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Biological Assay
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Biosensing Techniques / methods*
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Cell Cycle / drug effects
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Cell Proliferation / drug effects
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Cyclin A / antagonists & inhibitors
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Cyclin A / chemistry*
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Cyclin A / metabolism
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Cyclin-Dependent Kinases / antagonists & inhibitors
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Cyclin-Dependent Kinases / metabolism
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Fluorescent Dyes / chemistry
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Fluorescent Dyes / metabolism
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HeLa Cells
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Humans
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Models, Molecular
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Molecular Imprinting / methods*
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Neoplasms / drug therapy
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Neoplasms / metabolism*
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Peptide Library
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Peptides / chemical synthesis
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Peptides / metabolism*
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Phthalimides / chemistry*
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Phthalimides / metabolism
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Protein Binding
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Protein Kinase Inhibitors / metabolism
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Protein Kinase Inhibitors / pharmacology
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Spectrometry, Fluorescence
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Staining and Labeling / methods*
Substances
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Cyclin A
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Fluorescent Dyes
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Peptide Library
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Peptides
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Phthalimides
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Protein Kinase Inhibitors
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Cyclin-Dependent Kinases