Fluorescence in situ hybridization for phytoplasma and endophytic bacteria localization in plant tissues

Daniela Bulgari; Paola Casati; Franco Faoro

doi:10.1016/j.mimet.2011.08.001

Fluorescence in situ hybridization for phytoplasma and endophytic bacteria localization in plant tissues

J Microbiol Methods. 2011 Nov;87(2):220-3. doi: 10.1016/j.mimet.2011.08.001. Epub 2011 Aug 7.

Authors

Daniela Bulgari¹, Paola Casati, Franco Faoro

Affiliation

¹ Dipartimento di Produzione Vegetale, Università degli Studi di Milano, Via Celoria 2, 20133 Milano, Italy.

PMID: 21851838
DOI: 10.1016/j.mimet.2011.08.001

Abstract

In the present study, we developed a rapid and efficient fluorescence in situ hybridization assay (FISH) in non-embedded tissues of the model plant Catharanthus roseus for co-localizing phytoplasmas and endophytic bacteria, opening new perspectives for studying the interaction between these microorganisms.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Bacteria / classification
Bacteria / genetics
Bacteria / isolation & purification*
Endophytes / classification
Endophytes / genetics
Endophytes / isolation & purification*
In Situ Hybridization, Fluorescence / methods*
Phytoplasma / classification
Phytoplasma / genetics*
Phytoplasma / isolation & purification
Vinca / microbiology*