Abstract
Neuropilin-1 is a member of the neuropilins family protein, which contains a large extracellular domain (a1a2, b1b2 and c), a single transmembrane domain, and a short cytoplasmic tail. NRP-1 plays a critical role in angiogenesis and stimulates endothelial cell division and migration by binding VEGF(165) with b1b2 domain. In the present study, we report the establishment of a monoclonal antibody (A6-26-11-26 clone) specific for NRP-1 b1b2 through hybridoma method. Western blot analysis indicates that our NRP-1 b1b2 MAb can combine both NRP-1 b1b2 and NRP-1 originated from tumor cells. This monoclonal antibody against NRP-1 b1b2 will be useful in the further development of cancer target strategy.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal, Murine-Derived / chemistry
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Antibodies, Monoclonal, Murine-Derived / isolation & purification*
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Antibodies, Monoclonal, Murine-Derived / metabolism
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Antibody Specificity
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Blotting, Western
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Cells, Cultured
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Cloning, Molecular
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Escherichia coli / genetics
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Female
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Genetic Vectors
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Humans
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Hybridomas / metabolism
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Mice
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Mice, Inbred BALB C
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Neuropilin-1 / chemistry
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Neuropilin-1 / immunology*
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Neuropilin-1 / metabolism
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Protein Structure, Tertiary
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Recombinant Proteins / chemistry
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Recombinant Proteins / immunology*
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Recombinant Proteins / metabolism
Substances
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Antibodies, Monoclonal, Murine-Derived
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Recombinant Proteins
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Neuropilin-1