Carbon-13 labelling strategy for studying the ATP metabolism in individual yeast cells by micro-arrays for mass spectrometry

Pawel L Urban; A Mareike Schmidt; Stephan R Fagerer; Andrea Amantonico; Alfredo Ibañez; Konstantins Jefimovs; Matthias Heinemann; Renato Zenobi

doi:10.1039/c1mb05248a

Carbon-13 labelling strategy for studying the ATP metabolism in individual yeast cells by micro-arrays for mass spectrometry

Mol Biosyst. 2011 Oct;7(10):2837-40. doi: 10.1039/c1mb05248a. Epub 2011 Aug 18.

Authors

Pawel L Urban¹, A Mareike Schmidt, Stephan R Fagerer, Andrea Amantonico, Alfredo Ibañez, Konstantins Jefimovs, Matthias Heinemann, Renato Zenobi

Affiliation

¹ Department of Chemistry and Applied Biosciences, ETH Zurich, Switzerland.

PMID: 21850345
DOI: 10.1039/c1mb05248a

Abstract

Isotopic labelling of cellular metabolites, used in conjunction with high-density micro-arrays for mass spectrometry enables observation of ATP metabolism in single yeast cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Triphosphate / metabolism*
Carbon Isotopes
Saccharomyces cerevisiae / metabolism*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

Carbon Isotopes
Adenosine Triphosphate