This study investigated the effects of delayed access to feed and water on early duckling growth, selected aspects of intestinal physiology, and immune responses. Ducklings were assigned to one of 2 experimental groups. In one treatment group (referred to as the fed group), feed and water were provided beginning on d 0, and in the other treatment group (referred to as the withheld group), feed and water were withheld until d 2. The ducklings in the withheld treatment had lower BW at 2 and 6 d posthatch than ducklings in the fed group. At 8 d posthatch (6 d postfeeding), the BW of the ducklings in the withheld group was comparable to the BW of the 6-d-old ducklings in the fed group. At 2 d posthatch, withheld ducklings had lower absolute and relative duodenum plus pancreas weight than fed ducklings. At 8 d posthatch (6 d postfeeding), withheld ducklings had higher absolute and relative duodenum plus pancreas weight than fed ducklings at 6 d posthatch. At 2 d posthatch, mucin 5B mRNA content was approximately 2 times lower in withheld ducklings (P = 0.09) than in fed ducklings. At 6 d posthatch, mucin 5b mRNA content was approximately 2.5-fold higher in withheld ducklings (P = 0.07) than in fed ducklings. Delayed access to feed and water increased the CD25(+) cell number in the cecal tonsil at 2, 6, and 8 d posthatch. The IL-10 content of CD25(+) cells was higher in the withheld ducklings than in the fed ducklings at 2 and 6 d posthatch. In conclusion, delaying access of ducklings with no experimental pathogen infection to feed and water has no long-term effects on early growth parameters, intestinal physiology, and immune responses.