Influence of temperature and period of freezing on the generation of hemolysate and blood cell lysate

Abstract

Background: There is little information on temperature and period of freezing for generating hemolysate and blood cell lysate.

Materials and methods: Primary tubes containing whole blood were frozen at -20°C and -80°C and then serially thawed to assess cell lysis and clinical chemistry tests in centrifuged plasma.

Results and conclusions: Massive amount of blood cells lysis could only be obtained by storing samples 12h at -20°C, or 2h at -80°C.