Abstract
In the present study, the effect of a borneol/menthol eutectic mixture (25:75) and microemulsion on the absorption of daidzein in rat intestinal membrane was evaluated. The microemulsion formulation was composed of ethyl oleate (oil), Cremophor RH40 (surfactant), PEG400 (co-surfactant), and water. The borneol/menthol eutectic mixture and its microemulsion were found to enhance the intestinal absorption of daidzein in vitro. A diffusion chamber system with isolated rat intestinal membranes was used. In contrast, verapamil (0.3 mM), a typical P-glycoprotein inhibitor, showed no effect on the absorption of daidzein by this system. A pharmacokinetic study was conducted in rats. After oral administration of daidzein at a dose of 10 mg/kg in the form of either borneol/menthol eutectic mixtures or suspension, the relative bioavailability of borneol/menthol eutectic mixtures and microemulsion was enhanced by about 1.5- and 3.65-fold, respectively, compared with a daidzein suspension. In conclusion, a borneol/menthol eutectic mixture can enhance the absorption of daidzein, although the mechanism of absorption enhancement is still unclear.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / antagonists & inhibitors
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism
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Administration, Oral
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Animals
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Biological Availability
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Camphanes / administration & dosage
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Camphanes / chemistry
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Camphanes / pharmacology*
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Chemistry, Pharmaceutical
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Dose-Response Relationship, Drug
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Drug Compounding
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Emulsions
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Intestinal Absorption / drug effects*
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Intestinal Mucosa / metabolism
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Intestines / drug effects*
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Isoflavones / administration & dosage
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Isoflavones / chemistry
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Isoflavones / pharmacokinetics*
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Male
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Methanol / administration & dosage
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Methanol / chemistry
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Methanol / pharmacology*
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Oils / chemistry
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Oleic Acids / chemistry
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Polyethylene Glycols / chemistry
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Rats
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Rats, Sprague-Dawley
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Solubility
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Surface-Active Agents / chemistry
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Technology, Pharmaceutical
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Verapamil / pharmacology
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Water / chemistry
Substances
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ATP Binding Cassette Transporter, Subfamily B, Member 1
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Camphanes
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Emulsions
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Isoflavones
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Oils
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Oleic Acids
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Surface-Active Agents
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Water
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cremophor
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Polyethylene Glycols
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daidzein
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Verapamil
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isoborneol
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Methanol
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ethyl oleate