A near-infrared fluorescent calcium probe: a new tool for intracellular multicolour Ca2+ imaging

Chem Commun (Camb). 2011 Oct 7;47(37):10407-9. doi: 10.1039/c1cc14045k. Epub 2011 Aug 12.

Abstract

We report a novel near-infrared fluorescent calcium probe (KFCA), which has good optical properties such as intense NIR fluorescence emission (670 nm, QY: 0.24), excellent ON/OFF ratio (120-fold), and good wavelength-compatibility with visible-light-emissive fluorophores (Fluo-4, DsRed2), and which is applicable for real-time dual-colour intracellular Ca(2+) imaging.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Boron Compounds / chemistry
  • Calcium / metabolism*
  • Color
  • Fluorescent Dyes / chemistry*
  • HeLa Cells
  • Humans
  • Infrared Rays*
  • Intracellular Space / metabolism*
  • Molecular Imaging / methods*
  • Optical Phenomena

Substances

  • 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
  • Boron Compounds
  • Fluorescent Dyes
  • Calcium