Supravital fluorometric apoptosis detection in a single mouse embryo using lab-on-a-chip

Lab Chip. 2011 Oct 7;11(19):3263-8. doi: 10.1039/c0lc00667j. Epub 2011 Aug 11.

Abstract

Detection of apoptosis is one of the main criteria of preimplantation embryo growth potential assessment. Recent developments in lab-on-a-chip techniques has led to apoptosis detection and monitoring on a single cell or embryo level. However, single embryo apoptosis detection without a change in embryo developmental competence and post-examination "recovery" still remains a challenge. In this paper we present a lab-on-a-chip, co-working with miniaturized optical instrumentation, which allows supravital examination of single embryos for the presence of apoptotic blastomers with full after lab-on-a-chip study "recovery" and maintenance of their further developmental capacity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis*
  • Embryo, Mammalian / cytology*
  • Fluorometry / methods*
  • Lab-On-A-Chip Devices
  • Mice
  • Miniaturization