A simple and effective SuperBuffer for DNA agarose electrophoresis

Gene. 2011 Nov 1;487(1):72-4. doi: 10.1016/j.gene.2011.05.018. Epub 2011 May 30.

Abstract

In the paper, we describe a unique effective electrophoresis buffer for DNA agarose electrophoresis, called SuperBuffer. Using this buffer, electrophoresis could be performed within 10 min at voltages as high as 25V/cm. In addition, DNA fragments of different lengths could be isolated clearly even at lower agarose gel concentrations and the DNA recovery efficiency was higher than that of the TAE/TBE running buffers. The SuperBuffer still retained its electrophoretic effect even after several uses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetates
  • Boric Acids
  • Buffers
  • DNA / analysis*
  • Edetic Acid
  • Electrophoresis, Agar Gel / methods*
  • Ethylenediamines
  • Gels / chemistry
  • Reproducibility of Results
  • Sepharose / chemistry
  • Time Factors
  • Tromethamine

Substances

  • Acetates
  • Boric Acids
  • Buffers
  • Ethylenediamines
  • Gels
  • Tris-borate-EDTA buffer
  • tris-acetate-EDTA buffer
  • Tromethamine
  • DNA
  • Sepharose
  • Edetic Acid