Development of microsatellite markers for Euryale ferox (Nymphaeaceae), an endangered aquatic plant species in Japan

Am J Bot. 2011 Aug;98(8):e233-5. doi: 10.3732/ajb.1100056.

Abstract

Premise of the study: Microsatellite markers were developed to investigate genetic diversity and genetic structure of Euryale ferox, a vulnerable aquatic plant.

Methods and results: Ten microsatellite loci were isolated and characterized for this species. Eight loci showed polymorphisms with two or three alleles per locus. As for the polymorphic markers, the expected and observed heterozygosities ranged from 0.00 to 0.08 and 0.17 to 0.64, respectively.

Conclusions: The 10 microsatellite markers described here will be useful for investigating genetic diversity, genetic structure, and gene flow between populations of E. ferox.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Cloning, Molecular
  • DNA, Plant / analysis*
  • DNA, Plant / genetics
  • Endangered Species*
  • Gene Flow
  • Gene Frequency
  • Gene Library
  • Genetic Carrier Screening / methods
  • Genome, Plant*
  • Heterozygote
  • Japan
  • Microsatellite Repeats*
  • Nymphaeaceae / genetics*
  • Nymphaeaceae / metabolism
  • Plant Leaves / genetics
  • Plasmids / genetics
  • Plasmids / metabolism
  • Polymorphism, Genetic
  • Sequence Analysis, DNA
  • Species Specificity

Substances

  • DNA, Plant