A qualitative and quantitative investigation into the effect of fluoride formulations on enamel erosion and erosion-abrasion in vitro

J Dent. 2011 Oct;39(10):648-55. doi: 10.1016/j.jdent.2011.07.006. Epub 2011 Jul 28.

Abstract

Objectives: To investigate the effect of a single application of highly concentrated SnF(2) and NaF solutions and a NaF/CaF(2) varnish on human enamel subjected to hydrochloric acid erosion and tooth brush abrasion.

Methods: Forty enamel samples were prepared from human third molars and NaF (9500ppm, pH 8.0), SnF(2) (9500ppm, pH 2.6) solutions; Bifluorid10(®) varnish (42,500ppm, NaF 5%, CaF(2) 5%) and deionized water (control) was applied to the enamel. Following this three, six and nine cycles of erosion [1 cycle=erosion (0.01M HCl, pH 2.2, 2min)+artificial saliva (1h, pH 7.0)] and erosion-abrasion [1 cycle=erosion (0.01M HCl, pH 2.2, 2min)+artificial saliva (1h, pH 7.0)+abrasion (120 linear strokes in artificial saliva from Tepe medium soft brushes 200g loading)] were carried out. The fluoride treated enamel was analysed using Knoop microhardness, scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS).

Results: For erosion alone, there was significantly less microhardness reduction in the Bifluorid10(®) group after three and six cycles of erosion (P<0.05), however no other groups showed statistically different hardness (P>0.05). The EDS analysis showed that only the Bifluorid10(®) group had any detectable fluorine following erosion and erosion-abrasion (0.1wt.% and 0.2wt.% fluorine respectively). The surface fluorine was found to have been removed after erosion and erosion-abrasion for all other surface treatments. Although precipitates were observed after application of the surface treatments, following erosion-abrasion, no visible surface effects from any fluoride preparation remained.

Conclusions: Enamel surface precipitates from application NaF, SnF(2) solutions appear to not be able to provide protection against gastric erosion and tooth brush abrasion. The NaF/CaF(2) varnish provided limited protection against erosion but the role for such varnishes in gastric erosion and tooth brush abrasion remains uncertain.

MeSH terms

  • Analysis of Variance
  • Calcium Fluoride / administration & dosage
  • Dental Stress Analysis
  • Fluorides, Topical / administration & dosage*
  • Fluorine / analysis
  • Gastric Acid
  • Hardness
  • Humans
  • Hydrochloric Acid / adverse effects
  • Sodium Fluoride / administration & dosage
  • Spectrometry, X-Ray Emission
  • Tin Fluorides / administration & dosage
  • Tooth Abrasion / etiology
  • Tooth Abrasion / prevention & control*
  • Tooth Erosion / chemically induced
  • Tooth Erosion / prevention & control*
  • Toothbrushing / adverse effects

Substances

  • Bifluorid 12
  • Fluorides, Topical
  • Tin Fluorides
  • Fluorine
  • Sodium Fluoride
  • Calcium Fluoride
  • Hydrochloric Acid