Abstract
Primary immunization of humans with smallpox vaccine (live vaccinia virus (VACV)) consistently elicits antibody responses to six VACV virion membrane proteins, including A13. However, whether anti-A13 antibody contributes to immune protection against orthopoxviruses was unknown. Here, we isolated a murine monoclonal antibody (mAb) against A13 from a mouse that had been infected with VACV. The anti-A13 mAb bound to recombinant A13 protein with an affinity of 3.4nM and neutralized VACV mature virions. Passive immunization of mice with the anti-A13 mAb protected against intranasal VACV infection. The epitope of the anti-A13 mAb was mapped to a 10-amino acid sequence conserved in all orthopoxviruses, including viriola virus and monkeypox virus, suggesting that anti-A13 antibodies elicited by smallpox vaccine might contribute to immune protection against orthopoxviruses. In addition, our data demonstrates that anti-A13 mAbs are effective for treating orthopoxvirus infection.
Copyright © 2011 Elsevier Inc. All rights reserved.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Monoclonal / administration & dosage
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Antibodies, Monoclonal / immunology
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Antibodies, Monoclonal / isolation & purification
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Antibodies, Neutralizing / administration & dosage
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Antibodies, Neutralizing / immunology*
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Antibodies, Neutralizing / isolation & purification
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Antibodies, Viral / administration & dosage
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Antibodies, Viral / immunology*
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Antibodies, Viral / isolation & purification
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Body Weight
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Conserved Sequence
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Disease Models, Animal
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Epitope Mapping
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Epitopes, B-Lymphocyte / genetics
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Epitopes, B-Lymphocyte / immunology*
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Immunization, Passive
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Monkeypox virus / genetics
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Neutralization Tests
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Rodent Diseases / prevention & control
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Survival Analysis
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Vaccinia / prevention & control
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Vaccinia virus / genetics
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Vaccinia virus / immunology*
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Variola virus / genetics
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Viral Envelope Proteins / genetics
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Viral Envelope Proteins / immunology*
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Viral Plaque Assay
Substances
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Antibodies, Monoclonal
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Antibodies, Neutralizing
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Antibodies, Viral
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Epitopes, B-Lymphocyte
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Viral Envelope Proteins