Abstract
In this study, polyaniline-multiwalled carbon nanotube film (PANi-MWCNT) has been polymerized on interdigitated platinum electrode arrays (IDA), fabricated by MEMS technology for the detection of human papillomavirus (HPV) infection, using immobilized peptide aptamers as affinity capture reagent. Label-free, electrochemical detection of the specific immune reaction between antigen peptide aptamer HPV-16-L1 (with a molecular weight of 1825 Da), the most common genotype in cytological normal women worldwide, and its specific antibody of HPV-16 (which is much bigger with molecular weight of ca. 150 kDa) on multifunctional PANi-MWCNT based arrays was reported. The most significant advantage of this technique consists of reagentless and multiple detection of antigen-antibody complex formation on well conducting IDA interface of PANi-MWCNT, without intermediate steps or any labeling reagents, as normally required in the previous works.
Copyright © 2011 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Aniline Compounds / chemistry*
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Antibodies, Viral / immunology
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Antigens, Viral / immunology
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Aptamers, Peptide / analysis
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Aptamers, Peptide / chemistry
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Aptamers, Peptide / immunology
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Capsid Proteins / chemistry
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Capsid Proteins / genetics
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Capsid Proteins / immunology
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Electrochemical Techniques / instrumentation
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Electrochemical Techniques / methods*
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Electrodes
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Female
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Human papillomavirus 16 / genetics
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Human papillomavirus 16 / immunology
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Humans
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Microscopy, Atomic Force
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Microscopy, Electron, Scanning
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Nanotubes, Carbon / chemistry*
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Nanotubes, Carbon / ultrastructure
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Oncogene Proteins, Viral / chemistry
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Oncogene Proteins, Viral / genetics
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Oncogene Proteins, Viral / immunology
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Papillomavirus Infections / diagnosis
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Papillomavirus Infections / immunology
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Papillomavirus Infections / virology
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Uterine Cervical Neoplasms / diagnosis
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Uterine Cervical Neoplasms / immunology
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Uterine Cervical Neoplasms / virology
Substances
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Aniline Compounds
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Antibodies, Viral
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Antigens, Viral
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Aptamers, Peptide
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Capsid Proteins
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Nanotubes, Carbon
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Oncogene Proteins, Viral
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polyaniline
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L1 protein, Human papillomavirus type 16