Detection of gene expression in mouse embryos and tissue sections

Edwina McGlinn; Jennifer H Mansfield

doi:10.1007/978-1-61779-210-6_10

Detection of gene expression in mouse embryos and tissue sections

Methods Mol Biol. 2011:770:259-92. doi: 10.1007/978-1-61779-210-6_10.

Authors

Edwina McGlinn¹, Jennifer H Mansfield

Affiliation

¹ Department of Genetics, Harvard Medical School, Boston, MA, USA. emcglinn@receptor.med.harvard.edu

PMID: 21805268
DOI: 10.1007/978-1-61779-210-6_10

Abstract

Analysis of gene expression patterns is central to the study of embryonic development. This chapter details methods for detecting gene expression in whole mouse embryos and in tissue sections. The most commonly used methods available in mouse are described and include mRNA in situ hybridization, immunohistochemistry, and detection of enzymatic and fluorescent protein reporters.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Cryoultramicrotomy
Embryo, Mammalian / cytology*
Embryo, Mammalian / metabolism*
Gelatin / metabolism
Gene Expression Profiling / methods*
Genes, Reporter / genetics
Histocytological Preparation Techniques / methods*
Immunohistochemistry
In Situ Hybridization
Mice
Paraffin / metabolism
RNA Probes / genetics

Substances

RNA Probes
Paraffin
Gelatin