Re(CO)(3)(H(2)O)(3)(+) binding to lysozyme: structure and reactivity

Metallomics. 2011 Sep;3(9):909-16. doi: 10.1039/c1mt00065a. Epub 2011 Aug 1.

Abstract

The reaction of Re(CO)(3)(H(2)O)(3)(+) with hen egg white lysozyme in aqueous solution results in a single covalent adduct. Both NMR spectroscopy and single crystal X-ray diffraction show that the rhenium tricarbonyl cation binds to His15 via replacement of one of the coordinated water molecules. The formation of this adduct does not greatly affect the structure of the protein.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Binding Sites
  • Coordination Complexes / chemistry
  • Coordination Complexes / metabolism*
  • Crystallography, X-Ray
  • Histidine / chemistry
  • Models, Molecular
  • Muramidase / chemistry
  • Muramidase / metabolism*
  • Nuclear Magnetic Resonance, Biomolecular
  • Organometallic Compounds / chemistry
  • Organometallic Compounds / metabolism*
  • Protein Binding
  • Rhenium / chemistry
  • Rhenium / metabolism*
  • Structure-Activity Relationship
  • Water / chemistry

Substances

  • Coordination Complexes
  • Organometallic Compounds
  • Water
  • Histidine
  • Rhenium
  • hen egg lysozyme
  • Muramidase