Abstract
Werner interacting protein 1 (WRNIP1) that is highly conserved from Escherichia coli to human was originally identified as a protein that interacts with the Werner syndrome responsible gene product (WRN). Here, human WRNIP1 and WRN are shown to bind to template-primer DNA, and WRNIP1, but not WRN, requires ATP for DNA binding. Under conditions of a limiting amount of WRN, WRNIP1 facilitated binding of WRN to DNA in a dose-dependent manner. However, WRNIP1 did not stimulate the DNA helicase activity of WRN, and WRN displaced pre-bound WRNIP1 from DNA. Functional relationships between WRNIP1 and WRN will be discussed.
MeSH terms
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ATPases Associated with Diverse Cellular Activities
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Adenosine Triphosphate / metabolism
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Carrier Proteins / metabolism*
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DNA / metabolism*
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DNA Primers / metabolism*
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DNA-Binding Proteins / metabolism*
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Exodeoxyribonucleases / metabolism*
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Humans
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Protein Binding
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RecQ Helicases / metabolism*
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Werner Syndrome / genetics*
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Werner Syndrome / metabolism
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Werner Syndrome Helicase
Substances
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Carrier Proteins
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DNA Primers
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DNA-Binding Proteins
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Adenosine Triphosphate
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DNA
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Exodeoxyribonucleases
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WRNIP1 protein, human
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ATPases Associated with Diverse Cellular Activities
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RecQ Helicases
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WRN protein, human
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Werner Syndrome Helicase