New variants of E6 and E7 oncogenes of human papillomavirus type 31 identified in Northeastern Brazil

Bárbara S Chagas; Marcus V A Batista; Vilma Guimarães; Valdir Q Balbino; Sergio Crovella; Antonio C Freitas

doi:10.1016/j.ygyno.2011.07.008

New variants of E6 and E7 oncogenes of human papillomavirus type 31 identified in Northeastern Brazil

Gynecol Oncol. 2011 Nov;123(2):284-8. doi: 10.1016/j.ygyno.2011.07.008. Epub 2011 Jul 29.

Authors

Bárbara S Chagas¹, Marcus V A Batista, Vilma Guimarães, Valdir Q Balbino, Sergio Crovella, Antonio C Freitas

Affiliation

¹ Department of Genetics, Federal University of Pernambuco, Recife, PE, Brazil.

PMID: 21802716
DOI: 10.1016/j.ygyno.2011.07.008

Abstract

Objective: We sought to characterize E6 and E7 oncogenes genetic variability of HPV-31 isolated from cervical scraping samples of Northeastern Brazilian women.

Methods: E6 and E7 were amplified with specific primers, cloned and sequenced. The sequences obtained were aligned with the GenBank reference sequences with the aim of evaluating the possible genetic variants.

Results: We identified several genetic variants in E6 and E7 sequences from HPV-31 positive women. Three nucleotide changes in E6 were described for the first time in this study. Some nucleotide changes were non-synonymous substitutions.

Conclusion: The knowledge of region/country HPV specific genetic variations is relevant to understand the epidemiology and the development of effective vaccines.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Aged
Epitopes, B-Lymphocyte
Epitopes, T-Lymphocyte
Female
Genetic Variation
Human papillomavirus 31 / genetics*
Humans
Middle Aged
Oncogene Proteins, Viral / genetics*
Oncogene Proteins, Viral / immunology
Phylogeny
Uterine Cervical Neoplasms / virology*

Substances

Epitopes, B-Lymphocyte
Epitopes, T-Lymphocyte
Oncogene Proteins, Viral