Determination of gadolinium-based magnetic resonance imaging contrast agents by micellar electrokinetic capillary chromatography

Electrophoresis. 2011 Aug;32(16):2223-8. doi: 10.1002/elps.201100185. Epub 2011 Jul 27.

Abstract

MEKC with DAD was applied to detect six Gd-based contrasting agents (CAs) (Gd-DTPA-BMA (Omniscan), Gd-HPDO3A (ProHance), Gd-DOTA (Dotarem), Gd-AAZTA, Gd-BOPTA (Multihance) and Gd-DTPA (Magnevist)) commonly used in MRI diagnostics. The achieved LODs ranged between 0.40 and 20 μM and the optimized method gave excellent precision, especially when two internal standards were applied (less than 0.34 RSD% for migration time). The MEKC technique made it possible to determine the CAs in urine and serum samples of patients having a therapeutic dose. Due to the SDS content of the running buffer, the serum samples can be directly injected to analyze Gd-based CAs without interference of high protein content.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, Micellar Electrokinetic Capillary / methods*
  • Contrast Media / analysis
  • Contrast Media / chemistry*
  • Drug Monitoring
  • Gadolinium / blood
  • Gadolinium / chemistry*
  • Gadolinium / urine
  • Humans
  • Limit of Detection
  • Linear Models
  • Magnetic Resonance Imaging / methods*
  • Organometallic Compounds / blood
  • Organometallic Compounds / chemistry*
  • Organometallic Compounds / urine
  • Reproducibility of Results
  • Sodium Dodecyl Sulfate / chemistry

Substances

  • Contrast Media
  • Organometallic Compounds
  • Sodium Dodecyl Sulfate
  • Gadolinium