Investigation of anti-microbial activity of catechin on Escherichia coli growth by microcalorimetry

Dai Chunmei; Wang Jiabo; Kong Weijun; Peng Cheng; Xiao Xiaohe

doi:10.1016/j.etap.2010.08.002

Investigation of anti-microbial activity of catechin on Escherichia coli growth by microcalorimetry

Environ Toxicol Pharmacol. 2010 Nov;30(3):284-8. doi: 10.1016/j.etap.2010.08.002. Epub 2010 Aug 13.

Authors

Dai Chunmei¹, Wang Jiabo, Kong Weijun, Peng Cheng, Xiao Xiaohe

Affiliation

¹ Liaoning Medical University, Jinzhou 121001, PR China; China Military Institute of Chinese Materia Medica, 302 Military Hospital of China, Beijing 100039, PR China; Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, PR China.

PMID: 21787661
DOI: 10.1016/j.etap.2010.08.002

Abstract

Catechin is a major monomer of Chinese Rhubarb. Chinese Rhubarb has been claimed to have a therapeutic value as a bacteriostatic drug. It is also known that total rhubarb anthraquinone mixture and its individual components such as emodin are the effective components. However, the catechins component in Chinese Rhubarb such as catechin has not been characterized. In the present study, the power-time curves of Escherichia coli growth were obtained and the action on them by addition of catechin in different concentrations in vitro were studied by microcalorimetry. The results suggest E. coli growth is inhibited by catechin in a dose-dependent manner starting from the concentration of 0.05 to 1.6mgmL(-1). The correspondence analysis reveals k(2) and P(2) are the significant parameters to evaluate the anti-microbial effect. Microcalorimetry is a useful tool to evaluate the anti-microbial effect with its sensitive and significant quantitative information.