Quercetin protects the rat kidney against oxidative stress-mediated DNA damage and apoptosis induced by lead

Chan-Min Liu; Jie-Qiong Ma; Yun-Zhi Sun

doi:10.1016/j.etap.2010.07.002

Quercetin protects the rat kidney against oxidative stress-mediated DNA damage and apoptosis induced by lead

Environ Toxicol Pharmacol. 2010 Nov;30(3):264-71. doi: 10.1016/j.etap.2010.07.002. Epub 2010 Jul 16.

Authors

Chan-Min Liu¹, Jie-Qiong Ma, Yun-Zhi Sun

Affiliation

¹ School of Life Science, Xuzhou Normal University, No. 101, Shanghai Road, Tangshan New Area, Xuzhou City 221116, Xuzhou City, PR China.

PMID: 21787659
DOI: 10.1016/j.etap.2010.07.002

Abstract

Quercetin, a flavonoid, effectively improved the lead-induced histology changes including structure damage and leukocyte infiltration in rat kidney. The present study was designed to explore the protective mechanism of quercetin against lead-induced oxidative DNA damage and apoptosis in rat kidney. We found that quercetin markedly decreased the ROS level and lowered the GSH/GSSG ratio in the kidney of lead-treated rat. The increase of 8-hydroxydeoxyguanosine level in the kidney of lead-treated rat was effectively suppressed by quercetin. Furthermore, quercetin markedly restored Cu/Zn-SOD, CAT and GPx activities in the kidney of lead-treated rat. TUNEL assay showed that lead-induced apoptosis in rat kidney was significantly inhibited by quercetin, which might be attributed to its antioxidant property. In conclusion, these results suggested that quercetin could protect the rat kidney against lead-induced injury by improving renal function, attenuating histopathologic changes, reducing ROS production, renewing the activities of antioxidant enzymes, decreasing DNA oxidative damage and apoptosis.