One- and two-photon live cell imaging using a mutant SNAP-Tag protein and its FRET substrate pairs

Chong-Jing Zhang; Lin Li; Grace Y J Chen; Qing-Hua Xu; Shao Q Yao

doi:10.1021/ol201430x

One- and two-photon live cell imaging using a mutant SNAP-Tag protein and its FRET substrate pairs

Org Lett. 2011 Aug 19;13(16):4160-3. doi: 10.1021/ol201430x. Epub 2011 Jul 25.

Authors

Chong-Jing Zhang¹, Lin Li, Grace Y J Chen, Qing-Hua Xu, Shao Q Yao

Affiliation

¹ Department of Chemistry, National University of Singapore, Singapore 117543.

PMID: 21786765
DOI: 10.1021/ol201430x

Abstract

A small molecule-assisted protein labeling strategy based on a mutant SNAP-Tag (mSNAP) and its FRET substrate pairs has been developed. Both one- and two-photon fluorescence microscopic experiments were successfully demonstrated in living cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
CHO Cells
Cell Survival
Cricetinae
Cricetulus
Fluorescence Resonance Energy Transfer*
Photons
Proteins / analysis*
Proteins / genetics

Substances

Proteins