Analysis of mitochondrial 16S rRNA sequences of four speciments from two lizard species (Leiolepis guentherpetersi and L. reevesii) showed identity of 91.1-91.6% and the genetic distances were 8.8-9.3%. The two speciments (C5 and C7) of L. reevesii species have the homology of 96.5-99.4% with L. belliana and L. reevesii, respectively. Whereas, those of L. guentherpetersi species (S4 and S6) have higher homology of 99.6-100% with L. guttata and L. guentherpetersi, respectively. These mitochondrial 16S rRNA sequences of individuals from L. guentherpetersi (S4 and S6) and L. reevesii (C5 and C7) were deposited in GenBank with accession number EU428186, EU428187, EU428188, and EU428189, respectively.