Determination of DNA based on localized surface plasmon resonance light scattering using unmodified gold bipyramids

Spectrochim Acta A Mol Biomol Spectrosc. 2011 Oct 15;81(1):769-73. doi: 10.1016/j.saa.2011.07.023. Epub 2011 Jul 8.

Abstract

The DNA was determined based on resonance light scattering (RLS) spectrometry and the localized surface plasmon resonance. The gold bipyramids were used as the probes and synthesized by a seed-mediated method. Cetyltrimethylammonium bromide was used as stabilizing agent. DNA can be bound to the gold bipyramids due to electrostatic interaction and aggregates, which results in a strong enhancement of the RLS intensity. Under the optimal conditions, the intensity of RLS is directly proportional to the concentration of DNA in the range from 0.1 to 2.0 μg mL(-1).

Publication types

  • Evaluation Study

MeSH terms

  • Animals
  • Cetrimonium
  • Cetrimonium Compounds / chemistry
  • Cetrimonium Compounds / pharmacology
  • DNA / analysis*
  • Dose-Response Relationship, Drug
  • Excipients / chemistry
  • Excipients / pharmacology
  • Fishes / genetics
  • Fishes / metabolism
  • Gold / chemistry*
  • Gold / pharmacology
  • Hydrogen-Ion Concentration
  • Light*
  • Male
  • Metal Nanoparticles / chemistry
  • Microscopy, Electron, Transmission
  • Particle Size
  • Scattering, Radiation*
  • Spermatozoa / metabolism
  • Surface Plasmon Resonance / methods*
  • Time Factors

Substances

  • Cetrimonium Compounds
  • Excipients
  • Gold
  • DNA
  • Cetrimonium