Evaluation of chemopreventive activity of glutamine by the comet and the micronucleus assay in mice's peripheral blood

Rodrigo Juliano Oliveira; Evelyn Baise; Mariana de Oliveira Mauro; João Renato Pesarini; Renata Matuo; Ariane Fernanda da Silva; Lúcia Regina Ribeiro; Mário Sérgio Mantovani

doi:10.1016/j.etap.2009.03.008

Evaluation of chemopreventive activity of glutamine by the comet and the micronucleus assay in mice's peripheral blood

Environ Toxicol Pharmacol. 2009 Jul;28(1):120-4. doi: 10.1016/j.etap.2009.03.008. Epub 2009 Mar 24.

Authors

Rodrigo Juliano Oliveira¹, Evelyn Baise, Mariana de Oliveira Mauro, João Renato Pesarini, Renata Matuo, Ariane Fernanda da Silva, Lúcia Regina Ribeiro, Mário Sérgio Mantovani

Affiliation

¹ Departamento de Nutrição, Centro Universitário Filadélfia (UniFil), Londrina (PR), Brazil; Departamento de Biomedicina, Centro Universitário Filadélfia (UniFil), Londrina (PR), Brazil; CENUGEN - Centro de Estudos em Nutrição e Genética Toxicológica, Centro Universitário Filadélfia (UniFil), Londrina (PR), Brazil; Departamento de Biologia Geral, Universidade Estadual de Londrina (UEL), Londrina (PR), Brazil; Programa de Pós-graduação em Biologia Celular e Molecular, Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), Rio Claro (SP), Brazil.

PMID: 21783991
DOI: 10.1016/j.etap.2009.03.008

Abstract

This research has evaluated the effects of enteral supplementation of glutamine in clastogens and genotoxic damages caused by the acute administration of cisplatin. For this, it was utilized Swiss mice distributed in eight experimental groups: control, cisplatin, glutamine, in three different doses and the combination of these with cisplatin. The results show that the glutamine was present in neither genotoxic nor mutagenic activity. When in association with glutamine and cisplatin, in simultaneous treatment, it was verified the frequency decreased of micronuclei and comets. The damage reduction percentages to the micronucleus ranged from 95.4 to 91.8% after 24h of administration of these compounds and 76.7 to 56.8% after 48h. In the same time the damage reduction percentages to the comet test ranged from 117.0 to 115.0%. The results suggest that glutamine is capable of preventing genotoxic and mutagenic damage according to the experimental design proposed.