Proteomic investigation of 1,6-dimethoxyhexane testicular toxicity

Guillaume Pelletier; Sheila Masson; Yuan-Ling Wang; Michael G Wade; Susantha Mohottalage; Premkumari Kumarathasan; Renaud Vincent; Raymond Poon; Ih Chu

doi:10.1016/j.etap.2007.04.001

Proteomic investigation of 1,6-dimethoxyhexane testicular toxicity

Environ Toxicol Pharmacol. 2007 Sep;24(2):129-33. doi: 10.1016/j.etap.2007.04.001. Epub 2007 Apr 19.

Authors

Guillaume Pelletier¹, Sheila Masson, Yuan-Ling Wang, Michael G Wade, Susantha Mohottalage, Premkumari Kumarathasan, Renaud Vincent, Raymond Poon, Ih Chu

Affiliation

¹ Systemic Toxicology and Pharmacokinetics Section, Environmental and Occupational Toxicology Division, Environmental Health Sciences Bureau, Health Canada.

PMID: 21783800
DOI: 10.1016/j.etap.2007.04.001

Abstract

The aliphatic ether 1,6-dimethoxyhexane (DMH) was previously identified as a testicular toxicant. Testis protein extracts from control and DMH-treated rats were subjected to two-dimensional gel electrophoresis for comparison of protein expression profiles. MALDI-ToF peptide mass fingerprinting of differentially expressed proteins resulted in the conclusive identification of heat shock-related 70kDa protein 2 (HSP70.2), 60kDa heat shock protein, mitochondrial precursor (HSP60) and protein disulfide isomerase A3 precursor (ERp60). The potential involvement of these proteins in chemically induced perturbation of spermatogenesis and their utility as biomarkers of testicular toxicity are discussed in light of the knowledge currently available from the literature.