Purpose: To isolate and cultivate dental cells from goat deciduous teeth ,and explore changes of its biological characters before and after induced-mineralization.
Methods: Pulp cells were cultivated with modified tissue block enzymolytic method, cell lineage in the second passage with SAB methods was checked out. Induced-mineralized cultivation was adopted in the fourth passage, some examinations were used to compare with normal cultivated cells: cell proliferative capality, mineralized ability test, cell morphology change, protein(OCN) expression level, related osteogenic genes(ALP,COL-I,OCN,OPN) expression.
Results: Modified tissue block enzymolytic method could culture better pulp cells derived from goat deciduous teeth. Immunohistochemical staining showed that pulp cells were from mesenchyma. MTT method showed that induced-mineralization pulp cells proliferated more slowly than un-induced cells. Compared with uninduced-mineralization cells, induced-mineralization cells had stronger ALP activity and Alizarin red staining rate, its proteins(OCN) and mineralized genes(ALP,OCN) expression were significantly upregulated.
Conclusions: Pulp cells can be cultivated derived from goat exfoliated deciduous teeth with modified tissue block enzymolytic method. Fourteen days after continuous induced-mineralization culture , pulp cells derived from the goat deciduous teeth might own the potential in differentiating to osteoblast and form bone-like tissue.