This chapter presents protocols not only used to investigate the effect of microtubule-targeting agents on microtubule dynamic instability parameters, but also their impact on loading +TIPs at microtubule plus ends. These agents can be considered either as drugs to analyze their pharmacological effects on microtubule dynamics and their subsequent functions or as tools to improve basic knowledge on the regulation of microtubule dynamics. Deciphering the complexes of proteins that regulate microtubule dynamic instability may lead to the discovery of new potential targets for therapy.