Abstract
SNARE proteins mediate membrane fusion between synaptic vesicles and the plasma membrane. A minimized peptide SNARE model system with reduced complexity was introduced combining the native SNARE transmembrane (TMD) and linker domains with artificial coiled-coil forming peptides. Specific membrane fusion initiated by coiled-coil recognition was shown by lipid and content mixing vesicle assays.
This journal is © The Royal Society of Chemistry 2011
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Crystallography, X-Ray
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Liposomes / chemistry
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Membrane Fusion
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Molecular Sequence Data
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Peptides / chemistry*
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Peptides / metabolism
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Protein Structure, Tertiary
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SNARE Proteins / chemistry*
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SNARE Proteins / metabolism
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Syntaxin 1 / chemistry
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Syntaxin 1 / metabolism
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Vesicle-Associated Membrane Protein 2 / chemistry
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Vesicle-Associated Membrane Protein 2 / metabolism
Substances
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Liposomes
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Peptides
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SNARE Proteins
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Syntaxin 1
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Vesicle-Associated Membrane Protein 2