Abstract
The present study evaluated the anticomplement effects of isolated compounds from Achyranthes japonica in the classical pathway of the complement system. Using column chromatography, three compounds: achyranthoside C dimethyl ester, achyranthoside C butyl dimethyl ester, and achyranthoside E dimethyl ester were isolated and evaluated for in vitro anticomplement activity. Achyranthoside C dimethyl ester showed the most potent inhibitory activity against the complement system, with 50% inhibitory concentrations (IC(50)) values of 26.2 μg/mL. This is the first report of anticomplement activity of isolated compounds from A. japonica.
Publication types
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Research Support, Non-U.S. Gov't
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Retracted Publication
MeSH terms
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Achyranthes / chemistry*
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Animals
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Chloroform / chemistry
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Chromatography, High Pressure Liquid
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Chromatography, Thin Layer
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Complement Inactivating Agents / isolation & purification
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Complement Inactivating Agents / pharmacology*
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Complement Pathway, Classical / drug effects*
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Complement Pathway, Classical / immunology
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Hemolysis / immunology
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Inhibitory Concentration 50
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Oleanolic Acid / analogs & derivatives*
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Oleanolic Acid / isolation & purification
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Oleanolic Acid / pharmacology*
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Plant Extracts / chemistry
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Plant Extracts / isolation & purification
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Rabbits
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Saponins / isolation & purification
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Saponins / pharmacology*
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Sheep
Substances
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Complement Inactivating Agents
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Plant Extracts
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Saponins
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Oleanolic Acid
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Chloroform