The role of 1-methylcyclopropene in lignification and expansin gene expression in peeled water bamboo shoot (Zizania caduciflora L.)

J Sci Food Agric. 2011 Nov;91(14):2679-83. doi: 10.1002/jsfa.4512. Epub 2011 Jul 18.

Abstract

Background: Water bamboo shoot (WBS) (Zizania caduciflora L.) is a fleshy aquatic vegetable susceptible to lignification. In this study, effects of 1-methylcyclopropene (1-MCP) anti-ethylene treatment on lignification of harvested peeled WBS were investigated.

Results: Peeled shoots were treated with 0.5 µL L(-1) 1-MCP for 20 h at 20 °C and then stored at 20 °C for up to 9 days. Sensory quality, lignin content and activities of the lignification-associated enzymes peroxidase (POD) and phenylalanine ammonia lyase (PAL) were evaluated. Expression of expansin (ZcExp) was also assessed. 1-MCP application maintained better sensory quality and inhibited the increase in lignin content. Lessened lignification was associated with reduced activities of POD and PAL. Moreover, 1-MCP-treated shoots showed lower expression of the ZcExp gene.

Conclusion: 1-MCP pretreatment suppressed the synthesis of lignin and thereby delayed lignification in peeled WBS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cyclopropanes / pharmacology*
  • Ethylenes / antagonists & inhibitors
  • Food Handling
  • Food Preservatives / pharmacology*
  • Gene Expression Regulation, Plant / drug effects*
  • Lignin / metabolism*
  • Molecular Sequence Data
  • Peroxidase / metabolism
  • Phenylalanine Ammonia-Lyase / metabolism
  • Pigmentation / drug effects
  • Plant Proteins / chemistry
  • Plant Proteins / genetics
  • Plant Proteins / metabolism*
  • Plant Shoots / drug effects*
  • Plant Shoots / enzymology
  • Plant Shoots / metabolism
  • Poaceae / drug effects*
  • Poaceae / enzymology
  • Poaceae / metabolism
  • Quality Control
  • RNA, Messenger / metabolism
  • RNA, Plant / metabolism
  • Sensation
  • Sequence Homology, Nucleic Acid

Substances

  • Cyclopropanes
  • Ethylenes
  • Food Preservatives
  • Plant Proteins
  • RNA, Messenger
  • RNA, Plant
  • expansin protein, plant
  • Lignin
  • ethylene
  • Peroxidase
  • Phenylalanine Ammonia-Lyase
  • 1-methylcyclopropene