Abstract
A novel streptogramin A, pleuromutilin, and lincosamide resistance determinant, Vga(E), was identified in porcine methicillin-resistant Staphylococcus aureus (MRSA) ST398. The vga(E) gene encoded a 524-amino-acid protein belonging to the ABC transporter family. It was found on a multidrug resistance-conferring transposon, Tn6133, which was comprised of Tn554 with a stably integrated 4,787-bp DNA sequence harboring vga(E). Detection of Tn6133 in several porcine MRSA ST398 isolates and its ability to circularize suggest a potential for dissemination.
MeSH terms
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ATP-Binding Cassette Transporters / genetics*
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Animals
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Anti-Bacterial Agents / pharmacology*
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Bacterial Proteins / genetics*
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Base Sequence
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DNA Transposable Elements*
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Diterpenes / pharmacology
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Drug Resistance, Multiple, Bacterial / genetics*
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Genes, Bacterial
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Lincosamides / pharmacology
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Methicillin-Resistant Staphylococcus aureus / drug effects
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Methicillin-Resistant Staphylococcus aureus / genetics*
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Methicillin-Resistant Staphylococcus aureus / metabolism
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Plasmids / drug effects
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Pleuromutilins
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Polycyclic Compounds
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Sequence Analysis, DNA
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Staphylococcal Infections / microbiology
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Streptogramin A / pharmacology
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Swine
Substances
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ATP-Binding Cassette Transporters
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Anti-Bacterial Agents
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Bacterial Proteins
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DNA Transposable Elements
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Diterpenes
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Lincosamides
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Polycyclic Compounds
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VgA protein, Staphylococcus aureus
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Streptogramin A