New transposon Tn6133 in methicillin-resistant Staphylococcus aureus ST398 contains vga(E), a novel streptogramin A, pleuromutilin, and lincosamide resistance gene

Antimicrob Agents Chemother. 2011 Oct;55(10):4900-4. doi: 10.1128/AAC.00528-11. Epub 2011 Jul 18.

Abstract

A novel streptogramin A, pleuromutilin, and lincosamide resistance determinant, Vga(E), was identified in porcine methicillin-resistant Staphylococcus aureus (MRSA) ST398. The vga(E) gene encoded a 524-amino-acid protein belonging to the ABC transporter family. It was found on a multidrug resistance-conferring transposon, Tn6133, which was comprised of Tn554 with a stably integrated 4,787-bp DNA sequence harboring vga(E). Detection of Tn6133 in several porcine MRSA ST398 isolates and its ability to circularize suggest a potential for dissemination.

MeSH terms

  • ATP-Binding Cassette Transporters / genetics*
  • Animals
  • Anti-Bacterial Agents / pharmacology*
  • Bacterial Proteins / genetics*
  • Base Sequence
  • DNA Transposable Elements*
  • Diterpenes / pharmacology
  • Drug Resistance, Multiple, Bacterial / genetics*
  • Genes, Bacterial
  • Lincosamides / pharmacology
  • Methicillin-Resistant Staphylococcus aureus / drug effects
  • Methicillin-Resistant Staphylococcus aureus / genetics*
  • Methicillin-Resistant Staphylococcus aureus / metabolism
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Plasmids / drug effects
  • Pleuromutilins
  • Polycyclic Compounds
  • Sequence Analysis, DNA
  • Staphylococcal Infections / microbiology
  • Streptogramin A / pharmacology
  • Swine

Substances

  • ATP-Binding Cassette Transporters
  • Anti-Bacterial Agents
  • Bacterial Proteins
  • DNA Transposable Elements
  • Diterpenes
  • Lincosamides
  • Polycyclic Compounds
  • VgA protein, Staphylococcus aureus
  • Streptogramin A

Associated data

  • GENBANK/FR772051