Laboratory evaluation of the CHROMagar KPC medium for identification of carbapenem-nonsusceptible Enterobacteriaceae

Jacob Moran Gilad; Yehuda Carmeli; David Schwartz; Shiri Navon-Venezia

doi:10.1016/j.diagmicrobio.2010.03.005

Laboratory evaluation of the CHROMagar KPC medium for identification of carbapenem-nonsusceptible Enterobacteriaceae

Diagn Microbiol Infect Dis. 2011 Aug;70(4):565-7. doi: 10.1016/j.diagmicrobio.2010.03.005.

Authors

Jacob Moran Gilad¹, Yehuda Carmeli, David Schwartz, Shiri Navon-Venezia

Affiliation

¹ Clinical Microbiology Laboratory, Tel Aviv Sourasky Medical Center, Affiliated to the Sackler School of Medicine, Tel Aviv University, Tel Aviv 64239, Israel. giladko@014.net.il

PMID: 21767716
DOI: 10.1016/j.diagmicrobio.2010.03.005

Abstract

CHROMagar KPC was evaluated with 53 carbapenem-nonsusceptible strains. KPC-producing Klebsiella pneumoniae and Enterobacter cloacae grew efficiently and yielded metallic blue colonies (100% sensitivity). Poor or no growth or atypical colony color were evident with KPC-producing Escherichia coli (71.4% sensitivity) and ertapenem-resistant KPC-nonproducing K. pneumoniae (72.7% sensitivity). CHROMagar KPC appears applicable for processing carbapenem-resistant Enterobacteriaceae surveillance specimens, but its performance might possibly be suboptimal in the presence of KPC-negative ertapenem-resistant strains or for detection of KPC-producing E. coli.

Publication types

Evaluation Study

MeSH terms

Anti-Bacterial Agents / pharmacology*
Carbapenems / pharmacology*
Culture Media / chemistry*
Drug Resistance, Bacterial*
Enterobacteriaceae / drug effects*
Enterobacteriaceae / isolation & purification*
Enterobacteriaceae Infections / microbiology
Humans
Sensitivity and Specificity

Substances

Anti-Bacterial Agents
Carbapenems
Culture Media