Abstract
Bacterial two-hybrid analysis identified the Staphylococcus aureus RNA degradosome-like complex to include RNase J1, RNase J2, RNase Y, polynucleotide phosphorylase (PNPase), enolase, phosphofructokinase, and a DEAD box RNA helicase. Results also revealed that the recently recognized RNase RnpA interacts with the S. aureus degradosome and that this interaction is conserved in other Gram-positive organisms.
Publication types
-
Research Support, N.I.H., Extramural
MeSH terms
-
Bacterial Proteins / genetics
-
Bacterial Proteins / metabolism*
-
DEAD-box RNA Helicases / genetics
-
DEAD-box RNA Helicases / metabolism
-
Endoribonucleases / genetics
-
Endoribonucleases / metabolism*
-
Models, Biological
-
Multienzyme Complexes / genetics
-
Multienzyme Complexes / metabolism*
-
Phosphofructokinases / genetics
-
Phosphofructokinases / metabolism
-
Phosphopyruvate Hydratase / genetics
-
Phosphopyruvate Hydratase / metabolism
-
Polyribonucleotide Nucleotidyltransferase / genetics
-
Polyribonucleotide Nucleotidyltransferase / metabolism*
-
Protein Binding
-
RNA Helicases / metabolism*
-
RNA, Messenger / metabolism*
-
Staphylococcus aureus / enzymology*
Substances
-
Bacterial Proteins
-
Multienzyme Complexes
-
RNA, Messenger
-
degradosome
-
Phosphofructokinases
-
Polyribonucleotide Nucleotidyltransferase
-
Endoribonucleases
-
DEAD-box RNA Helicases
-
RNA Helicases
-
Phosphopyruvate Hydratase