Abstract
The genus of Nocardiopsis is a new source of antibiotics, chemicals, and enzymes. Here we reported the development of a vector and host system in moderately halophilic Nocardiopsis via an oriT-mediated conjugation. By screening about 80 Nocardiopsis strains, 6 of them harbored 8 plasmids (18-80 kb). The complete nucleotide sequence of pSQ10 consisted of 18,219 bp, with 71.9% G + C content, encoding 17 open reading frames, 5 of them resembled those of Streptomyces plasmids. A rep locus (iteron within the gene) was identified for replication in Nocardiopsis sp. YIM 90083, and rep protein bound to its iteron sequence. This system may be useful for gene cloning and expression in Nocardiopsis.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Actinomycetales / classification
-
Actinomycetales / genetics*
-
Actinomycetales / metabolism
-
Anti-Bacterial Agents / pharmacology
-
Bacterial Proteins / genetics
-
Bacterial Proteins / metabolism
-
Base Composition / genetics
-
Base Sequence
-
DNA Replication*
-
DNA, Bacterial / chemistry
-
DNA, Bacterial / genetics
-
DNA, Bacterial / metabolism
-
Drug Resistance, Bacterial
-
Escherichia coli / genetics
-
Genetic Vectors / genetics*
-
Molecular Sequence Data
-
Open Reading Frames / genetics
-
Phylogeny
-
Plasmids / genetics*
-
Protein Binding
-
Replication Origin / genetics
-
Sequence Analysis, DNA
-
Species Specificity
-
Thiostrepton / pharmacology
-
Transformation, Bacterial
Substances
-
Anti-Bacterial Agents
-
Bacterial Proteins
-
DNA, Bacterial
-
Thiostrepton