New method for titration of virus infectivity by immunostaining

Viral Immunol. 1990 Fall;3(3):237-41. doi: 10.1089/vim.1990.3.237.

Abstract

We have developed a new method for titration of viruses utilizing automated microtiter technology. Compared to existing methods such as plaque assay or hemagglutination titration of influenza virus, the new method offers distinct advantages in terms of time and effort. In addition because multiple replicates can easily be employed accuracy can be increased.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Antigens, Viral / immunology
  • Cell Line
  • Evaluation Studies as Topic
  • Hemagglutination, Viral
  • Immunoenzyme Techniques*
  • Influenza A virus / pathogenicity
  • Mice
  • Mice, Inbred BALB C
  • Sensitivity and Specificity
  • Simplexvirus / pathogenicity
  • Vero Cells
  • Viral Plaque Assay
  • Virology / methods*
  • Viruses / pathogenicity*

Substances

  • Antibodies, Monoclonal
  • Antigens, Viral