Determination of luteolin and apigenin in celery using ultrasonic-assisted extraction based on aqueous solution of ionic liquid coupled with HPLC quantification

J Sci Food Agric. 2011 Dec;91(15):2888-92. doi: 10.1002/jsfa.4553. Epub 2011 Jul 11.

Abstract

Background: Ionic liquids are a class of organic salts with melting points equal to or below 100 °C, and their use in sample preparation has shown its promise, for example, in alleviation of environmental pollution and improvement of selectivity and extraction of certain interesting compounds.

Results: Optimal conditions were obtained by using 1.0 mol L(-1) 1-butyl-3-methylimidazolium methylsulfate ([Bmim][MS]) aqueous solution at pH 1.0 as the extraction solvent. An ultrasonic power of 200 W was chosen to ensure complete dispersion of [Bmim][MS] into celery in 90 min and the solid:liquid ratio was 1:10.

Conclusion: The proposed ultrasonic assistant extraction approach, based on ionic liquid aqueous solution, exhibited satisfactory results, with recovery values ranging from 72.7% to 89.5%. Under optimal conditions, compared with conventional solvent extraction amounts of luteolin and apigenin were more than doubled.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apigenin / isolation & purification*
  • Apium / chemistry*
  • Chemical Fractionation / methods*
  • Chromatography, High Pressure Liquid / methods
  • Hydrogen-Ion Concentration
  • Imidazoles
  • Ionic Liquids*
  • Luteolin / isolation & purification*
  • Plant Extracts / chemistry*
  • Plant Extracts / isolation & purification
  • Solutions
  • Solvents

Substances

  • 1-butyl-3-methylimidazolium
  • Imidazoles
  • Ionic Liquids
  • Plant Extracts
  • Solutions
  • Solvents
  • Apigenin
  • Luteolin