Abstract
Altered gene expression of the proto-oncogenes c-fos and c-myc is associated with differentiation of the human promyelocytic leukemia (HL-60) cells. We studied changes of cyclic AMP levels and c-fos and c-myc mRNA levels after stimulation with theophylline and theophylline plus isoproterenol. Reduced c-fos and c-myc mRNA levels were detected, but the reduction could not, however, be related to the observed alternations in cyclic AMP concentrations. Expression of c-jun and c-Ha-ras was not affected under these conditions.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Cyclic AMP / metabolism*
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Gene Expression / drug effects
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Humans
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Isoproterenol / pharmacology
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Leukemia, Promyelocytic, Acute
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Molecular Sequence Data
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Proto-Oncogene Proteins / genetics*
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Proto-Oncogene Proteins c-fos
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Proto-Oncogene Proteins c-myc / genetics*
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Proto-Oncogenes*
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RNA, Messenger / metabolism*
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Tetradecanoylphorbol Acetate / pharmacology
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Theophylline / pharmacology
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Tumor Cells, Cultured
Substances
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-fos
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Proto-Oncogene Proteins c-myc
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RNA, Messenger
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Theophylline
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Cyclic AMP
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Isoproterenol
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Tetradecanoylphorbol Acetate