Physiological cyclic AMP stimulation and oncogene mRNA levels in HL-60 cells

Life Sci. 1990;47(17):1555-60. doi: 10.1016/0024-3205(90)90184-s.

Abstract

Altered gene expression of the proto-oncogenes c-fos and c-myc is associated with differentiation of the human promyelocytic leukemia (HL-60) cells. We studied changes of cyclic AMP levels and c-fos and c-myc mRNA levels after stimulation with theophylline and theophylline plus isoproterenol. Reduced c-fos and c-myc mRNA levels were detected, but the reduction could not, however, be related to the observed alternations in cyclic AMP concentrations. Expression of c-jun and c-Ha-ras was not affected under these conditions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cyclic AMP / metabolism*
  • Gene Expression / drug effects
  • Humans
  • Isoproterenol / pharmacology
  • Leukemia, Promyelocytic, Acute
  • Molecular Sequence Data
  • Proto-Oncogene Proteins / genetics*
  • Proto-Oncogene Proteins c-fos
  • Proto-Oncogene Proteins c-myc / genetics*
  • Proto-Oncogenes*
  • RNA, Messenger / metabolism*
  • Tetradecanoylphorbol Acetate / pharmacology
  • Theophylline / pharmacology
  • Tumor Cells, Cultured

Substances

  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-fos
  • Proto-Oncogene Proteins c-myc
  • RNA, Messenger
  • Theophylline
  • Cyclic AMP
  • Isoproterenol
  • Tetradecanoylphorbol Acetate